کیت PCR آنزیم Add Taq DNA Polymerase به همراه بافر واکنش و MgCl2 محصول AddBio
کیت PCR آنزیم Add Taq DNA Polymerase به همراه بافر واکنش و MgCl2 محصول AddBio
توضیحات
: Components
Add Taq DNA Polymerase (5.0 U/ µl) 1,000 units, 200 µl -
10x Reaction Buffer (without Mg) 1.0 ml x 2 tubes -
25mM MgCl2 1.0 ml x 2 tubes -
: Storage Condition
℃Store at -20
: Description
Add Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the
thermophile, Thermus aquaticus
’The molecular weight of the recombinant protein is 94kD. Add Taq DNA polymerase catalyzes the 5’→3
synthesis of DNA but has no detectable 3’→5’ proofreading exonuclease activity, and possesses low
5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product
: Storage Buffer
20mM Tris-HCl (pH 8.0), 100mM KCl, 3mM MgCl2, 1mM DTT, 0.1% Nonidet P-40, 0.1% Tween® 20 and
50% (v/v) glycerol
: 10X Reaction Buffer
100mM Tris-HCl (pH8.8), 500mM KCl, 1% Triton® X-100 and 20mM MgCl2
: Storage and Stability
Add Taq DNA Polymerase is stable for 2 years when stored in a constant temperature freezer at less
℃than -20
Made in KOREA